Zheng Y, Xu S, Zhang B, Ren M, Wu Z
Department of Physiology, Jiangxi Medical College, Nanchang.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 May;13(2):171-3.
The effect of endothelin-1 (ET) on progesterone production of isolated rat granulosa cells and its mechanism were investigated. ET could inhibit the hCG-stimulated progesterone production significantly at the concentration of 10(-8) mol/L (P < 0.05, n = 6) and highly significantly at the concentration of 10(-7) mol/L (P < 0.05, n = 6), but ET did not inhibit the basal progesterone production at various concentrations (10(-10)-10(-7) mol/L). Further experiments showed that the inhibitory effect of ET on the hCG-stimulated progesterone production could be reversed by rabbit antiserum to ET (ET-A, 1:1000) or cAMP (10(5) mol/L). ET could decrease the binding sites and the affinity constant (ka) of LH/hCG receptor on granulosa cells. All these findings suggest that ET may be an intraovarian regulatory factor which may inhibit progesterone production stimulated by hCG from rat granulosa cells through inerfering LH/hCG receptor function and cAMP formation.
研究了内皮素-1（ET）对离体大鼠颗粒细胞孕酮分泌的影响及其机制。ET在浓度为10^(-8)mol/L时可显著抑制hCG刺激的孕酮分泌（P<0.05，n=6），在浓度为10^(-7)mol/L时抑制作用极显著（P<0.05，n=6），但在不同浓度（10^(-10)-10^(-7)mol/L）下ET均不抑制基础孕酮分泌。进一步实验表明，ET对hCG刺激的孕酮分泌的抑制作用可被抗ET兔抗血清（ET-A，1:1000）或cAMP（10^5mol/L）逆转。ET可降低颗粒细胞上LH/hCG受体的结合位点和亲和常数（ka）。所有这些发现提示，ET可能是一种卵巢内调节因子，它可能通过干扰LH/hCG受体功能和cAMP形成来抑制大鼠颗粒细胞中hCG刺激的孕酮分泌。
