Denkova R, Bourneva V, Baleva K, Yaneva E, Nikolov B, Christov I, Ivanov I
Institute of Experimental Morphology and Antropology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Endocr Regul. 2000 Sep;34(3):157-60.
To investigate the in vitro effect of endothelin-1 (ET-1) on the steroid production (progesterone [P] and estradiol [E2]) by cultured human granulosa cells (GCs) during aging.
Human ovarian GCs and granulosa-luteal cells (GLCs) were isolated from ovaries of female patients (young and premenopausal) undergoing surgery for non-ovarian benign gynecological conditions. Cells were cultured with ET-1 in the presence or in the absence of FSH. The concentrations of P and E2 in conditioned media were determined by means of RIA.
In human GCs and GLCs obtained from young and premenopausal women, ET-1 in vitro can significantly reduce the FSH stimulated biosynthesis of P, whereas the basal P biosynthesis is only insignificantly diminished. The in vitro application of ET-1 have only a sparse inhibitory effect on both the basal and FSH stimulated biosynthesis of E2 in GCs from the two patient groups.
Our findings support the opinion that ET-1 is a local regulator of ovarian steroidogenesis which might modulate the steroid production and the stimulatory effect of FSH in cultured GCs and GLCs obtained from women at various ages.
研究内皮素-1（ET-1）在体外对不同年龄段培养的人颗粒细胞（GCs）甾体激素生成（孕酮[P]和雌二醇[E2]）的影响。
从因非卵巢良性妇科疾病接受手术的女性患者（年轻和绝经前）卵巢中分离出人卵巢颗粒细胞和颗粒黄体细胞（GLCs）。细胞在有或无促卵泡激素（FSH）的情况下与ET-1一起培养。通过放射免疫分析法测定条件培养基中P和E2的浓度。
在从年轻和绝经前女性获得的人GCs和GLCs中，ET-1在体外可显著降低FSH刺激的P生物合成，而基础P生物合成仅略有减少。ET-1的体外应用对来自两组患者的GCs中基础和FSH刺激的E2生物合成仅有微弱的抑制作用。
我们的研究结果支持这样一种观点，即ET-1是卵巢甾体激素生成的局部调节因子，它可能调节从不同年龄段女性获得的培养GCs和GLCs中的甾体激素生成以及FSH的刺激作用。
