Aqvist J, Kolmodin K, Florian J, Warshel A
Department of Molecular Biology, Uppsala University Biomedical Center, Box 590, S-75124 Uppsala, Sweden.
Chem Biol. 1999 Mar;6(3):R71-80. doi: 10.1016/S1074-5521(99)89003-6.
Phosphate monoester hydrolysis reactions in enzymes and solution are often discussed in terms of whether the reaction pathway is associative or dissociative. Although experimental results for solution reactions have usually been considered as evidence for the second alternative, a closer thermodynamic analysis of observed linear free energy relationships shows that experimental information is consistent with the associative, concerted and dissociative alternatives.
酶和溶液中的磷酸单酯水解反应常常依据反应途径是缔合型还是解离型来进行讨论。尽管溶液反应的实验结果通常被视为支持第二种情况的证据，但对观察到的线性自由能关系进行更深入的热力学分析表明，实验信息与缔合型、协同型和解离型情况均相符。
