Yung M H, Schaffer R, Putterill J
School of Biological Sciences, University of Auckland, New Zealand.
Plant J. 1999 Jan;17(2):203-8. doi: 10.1046/j.1365-313x.1999.00359.x.
The floral homeotic gene AGAMOUS (AG) imparts carpel identity on the fourth whorl of floral organs in wild-type Arabidopsis flowers. Less is known about the genes that regulate carpel patterning and differentiation. To identify cndidate regulators, we screened for genes expressed in developing carpels. Since Arabidopsis carpels are difficult to isolate, we used whole inflorescence apices of two floral homeotic mutants (pi and pi ag) and mRNA differential display, to identify carpel transcripts. Two of the resulting cDNA clones were shown to be expressed predominantly in flowers. They encoded AGL11, a MADS box transcription factor known to be expressed in the carpel and ovules, and a novel Arabidopsis endo-1,4-beta-D-glucanase (ATCEL2). In situ hybridisation localised the ATCEL2 transcript to the developing septum and ovule primordia of young carpels.
花同源异型基因AGAMOUS（AG）赋予野生型拟南芥花器官第四轮心皮特性。关于调控心皮模式形成和分化的基因，人们了解较少。为了鉴定潜在的调控因子，我们筛选了在发育中心皮中表达的基因。由于拟南芥的心皮难以分离，我们使用了两个花同源异型突变体（pi和pi ag）的整个花序顶端以及mRNA差异显示技术，来鉴定心皮转录本。所得到的两个cDNA克隆显示主要在花中表达。它们编码AGL11，一种已知在心皮和胚珠中表达的MADS盒转录因子，以及一种新的拟南芥内切-1,4-β-D-葡聚糖酶（ATCEL2）。原位杂交将ATCEL2转录本定位到幼嫩心皮发育中的隔膜和胚珠原基。
