Chen Z, Jia W, Kaufman P L, Cynader M
Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
Curr Eye Res. 1999 Jan;18(1):39-48. doi: 10.1076/ceyr.18.1.39.5391.
To investigate the pattern of dopamine-beta-hydroxylase (DBH)-containing fibers in human and monkey eyes.
DBH-containing fibers were detected by immunohistochemistry. The primary antibody used recognized DBH, the key enzyme in the conversion of dopamine to noradrenaline.
In the anterior segment, DBH immunoreaction product was found in the peripheral corneal endothelium layer, in both the dilator and sphincter muscles of the iris, as well as in the anterior border layer of the iris. The ciliary muscle and the stroma of the ciliary processes were also zones of concentration. In the posterior segment, staining was seen around blood vessels in the choroid, in the vascular walls of the short posterior ciliary arteries and in the ciliary nerves. The retina was also immunopositive, with specific labeling in cones and rods of photoreceptors, inner and outer plexiform layers and ganglion cell layer. There was no significant difference in the distribution of DBH-related immunoreactivity in human and monkey eyes.
The localization of DBH-related immunoreactivity is generally consistent with the known physiological roles of noradrenaline. However, an apparently high concentration of the enzyme in the anterior border layer of the iris and in retinal photoreceptors raises questions about the possible role of DBH-containing fibers in these structures.
研究人和猴眼中含多巴胺-β-羟化酶（DBH）纤维的分布模式。
采用免疫组织化学方法检测含DBH的纤维。所用一抗识别DBH，它是多巴胺转化为去甲肾上腺素过程中的关键酶。
在前房段，DBH免疫反应产物见于周边角膜内皮细胞层、虹膜的瞳孔开大肌和瞳孔括约肌以及虹膜前缘层。睫状肌和睫状突基质也是免疫反应产物集中的区域。在后房段，脉络膜血管周围、后短睫状动脉血管壁和睫状神经有染色。视网膜也呈免疫阳性，在光感受器的视锥细胞和视杆细胞、内网状层和外网状层以及神经节细胞层有特异性标记。人和猴眼中DBH相关免疫反应性的分布无显著差异。
DBH相关免疫反应性的定位通常与去甲肾上腺素已知的生理作用一致。然而，虹膜前缘层和视网膜光感受器中该酶的明显高浓度引发了关于含DBH纤维在这些结构中可能作用的疑问。
