Chen Z, Gu Q, Kaufman P L, Cynader M S
Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
Curr Eye Res. 1998 Apr;17(4):370-9. doi: 10.1080/02713689808951217.
To localize nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) in normal human and monkey eyes.
NADPH-d activity in ocular tissue sections was revealed by histochemical staining.
NADPH-d staining was found in the anterior and posterior ocular segment of both species. In the anterior segment, reaction product was present in the corneal epithelium and endothelium, iris dilator and sphincter, lens capsule, ciliary muscle, and non-pigmented epithelial cells and stroma of the ciliary processes. Staining density in the longitudinal ciliary muscle was higher than in the other ciliary muscle regions in the human; such regionalization was less apparent in the monkey. The trabecular meshwork stained minimally if at all in both species. In the posterior segment, staining was seen around choroidal blood vessels and choroidal nerves, and in the retinal pigment epithelial, photoreceptor, bipolar, inner plexiform and ganglion cell layers.
NADPH-d is widespread in human and monkey eyes, indicating significant and presumably functionally relevant NO production in the various ocular structures.
在正常人和猴眼中定位烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）。
通过组织化学染色显示眼组织切片中的NADPH-d活性。
在两种动物的眼前节和后节均发现NADPH-d染色。在前节，反应产物存在于角膜上皮和内皮、虹膜开大肌和括约肌、晶状体囊、睫状肌以及睫状体的无色素上皮细胞和基质中。人纵向睫状肌的染色密度高于其他睫状肌区域；在猴中这种区域化不太明显。小梁网在两种动物中染色最少（如果有染色的话）。在后节，在脉络膜血管和脉络膜神经周围以及视网膜色素上皮、光感受器、双极、内网状层和神经节细胞层可见染色。
NADPH-d在人和猴眼中广泛分布，表明在各种眼结构中有显著且可能与功能相关的一氧化氮产生。
