Haubold E M, Aronson J F, Cowan D F, McGinnis M R, Cooper C R
Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.
Med Mycol. 1998 Oct;36(5):263-7.
Fungal-specific primers targeted for highly conserved genomic nucleic acid sequences were used in a polymerase chain reaction (PCR) to amplify DNA from lobomycosis lesions in a bottlenose dolphin. Sequence alignments of this DNA possessed high homology to fungal ribosomal DNA sequences found in the genus Cladosporium. When used for in situ hybridization, the riboprobe transcribed from a cloned PCR-generated fragment bound to Loboa loboi cells. These results support the hypothesis that L. loboi in dolphin tissue is a fungus.
针对高度保守的基因组核酸序列设计的真菌特异性引物，用于聚合酶链反应（PCR），以扩增宽吻海豚中芽生菌病病变组织的DNA。该DNA的序列比对结果显示，其与枝孢属真菌的核糖体DNA序列具有高度同源性。当用于原位杂交时，从克隆的PCR扩增片段转录而来的核糖探针可与罗博阿洛博伊氏菌细胞结合。这些结果支持了海豚组织中的罗博阿洛博伊氏菌是一种真菌的假说。
