烟草BY-2无细胞提取物可诱导失活的哺乳动物中心体的微管成核活性恢复。

Tobacco BY-2 cell-free extracts induce the recovery of microtubule nucleating activity of inactivated mammalian centrosomes.

作者信息

Stoppin-Mellet V, Peter C, Buendia B, Karsenti E, Lambert A M

机构信息

Institut de Biologie Moléculaire des Plantes du CNRS, UPR406, Université Louis Pasteur, 12 rue du Général Zimmer, 67084, Strasbourg Cedex, France.

出版信息

Biochim Biophys Acta. 1999 Feb 4;1449(1):101-6. doi: 10.1016/s0167-4889(98)00179-7.

DOI:10.1016/s0167-4889(98)00179-7

PMID:10076055

Abstract

The structure and the molecular composition of the microtubule-organizing centers in acentriolar higher plant cells remain unknown. We developed an in vitro complementation assay where tobacco BY-2 extracts can restore the microtubule-nucleating activity of urea-inactivated mammalian centrosomes. Our results provide first evidence that soluble microtubule-nucleating factors are present in the plant cytosolic fraction. The implication for microtubule nucleation in higher plants is discussed.

摘要

无中心粒高等植物细胞中微管组织中心的结构和分子组成仍然未知。我们开发了一种体外互补测定法，烟草BY - 2提取物能够恢复尿素灭活的哺乳动物中心体的微管成核活性。我们的结果首次证明了植物细胞质部分中存在可溶性微管成核因子。文中还讨论了其对高等植物微管成核的意义。