Gray A M, Flatt P R
School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland BT52 1SA, UK.
J Endocrinol. 1999 Mar;160(3):409-14. doi: 10.1677/joe.0.1600409.
Viscum album (mistletoe) has been documented as a traditional treatment of diabetes. In acute 20-min tests, 1-10 mg/ml aqueous extract of mistletoe evoked a stepwise 1.1- to 12.2-fold stimulation of insulin secretion from clonal pancreatic B-cells. This effect was abolished by 0.5 mM diazoxide and prior exposure to extract did not alter subsequent stimulation of insulin secretion induced by 10 mM L-alanine, thereby negating a detrimental effect on cell viability. The insulin-releasing effect of mistletoe extract was unaltered by 16.7 mM glucose, l-alanine (10 mM), 3-isobutyl-1-methylxanthine (IBMX) (1 mM), or a depolarising concentration of KCl (25 mM). The ability of extract to enhance insulin secretion did not depend upon the use of heat during extract preparation and was not mediated by lectins. These results demonstrate the presence of insulin-releasing natural product(s) in Viscum album which may contribute to the reported antidiabetic property of the plant.
欧洲槲寄生已被记载为糖尿病的一种传统治疗方法。在急性20分钟试验中，1 - 10毫克/毫升的槲寄生水提取物能使克隆胰腺β细胞的胰岛素分泌呈逐步的1.1至12.2倍刺激。这种作用被0.5毫摩尔二氮嗪消除，且预先暴露于提取物并不改变随后由10毫摩尔L - 丙氨酸诱导的胰岛素分泌刺激，从而排除了对细胞活力的有害影响。槲寄生提取物的胰岛素释放作用不受16.7毫摩尔葡萄糖、L - 丙氨酸（10毫摩尔）、3 - 异丁基 - 1 - 甲基黄嘌呤（IBMX）（1毫摩尔）或去极化浓度的氯化钾（25毫摩尔）影响。提取物增强胰岛素分泌的能力不依赖于提取物制备过程中是否加热，且不是由凝集素介导的。这些结果表明欧洲槲寄生中存在胰岛素释放天然产物，这可能有助于该植物所报道的抗糖尿病特性。
