Eno A E, Ofem O E, Nku C O, Ani E J, Itam E H
Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria.
Afr J Med Med Sci. 2008 Jun;37(2):141-7.
Twenty male white rats (250-300 g) of Wistar strain were randomly divided into two batches, the normoglycaemic batch and the streptozotocin-induced diabetic batch often rats each. Animals in each batch were further divided into two groups of five rats per group. After an overnight fast (12 hrs), animals in each group received D-glucose load (2.0 g/kg.i.v) under pentobarbital anaesthesia, with or without the crude extract (100 mg/kg/iv). Blood samples were collected intravenously at 15 min intervals for 3 hrs. for analysis of glucose, insulin and glucagon levels. From the results, the extract (100 mg/kg) did not appear to have any significant effect on the blood glucose level of normal rats, but produced about 35.3% decrease in the diabetic rats. Despite the apparent lack of action on glucose level of normal rats, the extract stimulated insulin secretion by about 92.9% (% control) in this group, and about 81.5% in the diabetic group (% control). The glucagon level was not altered by the extract in the normal rats. In the diabetic group, there was mild but significant suppression ofglucagon level after the first 1 hr. which lasted for about 50 min. We suggest that this extract from V. album leaves may possess antihyperglycaemic, insulinotropic, and possibly, mild glucagonostatic agent(s) and may therefore be a candidate for the anti-diabetic drugs.
将20只体重250 - 300克的雄性Wistar品系大白鼠随机分为两批，一批为正常血糖组，另一批为链脲佐菌素诱导的糖尿病组，每组各10只大鼠。每批动物再进一步分为两组，每组5只大鼠。禁食过夜（12小时）后，每组动物在戊巴比妥麻醉下接受D - 葡萄糖负荷（2.0克/千克，静脉注射），同时或不给予粗提取物（100毫克/千克，静脉注射）。每隔15分钟静脉采集血样，持续3小时，用于分析血糖、胰岛素和胰高血糖素水平。结果显示，提取物（100毫克/千克）对正常大鼠的血糖水平似乎没有显著影响，但能使糖尿病大鼠的血糖水平降低约35.3%。尽管提取物对正常大鼠的血糖水平明显没有作用，但在该组中它能刺激胰岛素分泌约92.9%（相对于对照组），在糖尿病组中能刺激约81.5%（相对于对照组）。提取物对正常大鼠的胰高血糖素水平没有改变。在糖尿病组中，最初1小时后胰高血糖素水平有轻微但显著的抑制，持续约50分钟。我们认为，这种白桂木叶提取物可能具有降血糖、促胰岛素分泌以及可能具有轻度抑制胰高血糖素的作用，因此可能是抗糖尿病药物的候选物。
