Marlovits T C, Zechmeister T, Schwihla H, Ronacher B, Blaas D
Institute of Biochemistry, University of Vienna, Austria.
J Mol Recognit. 1998 Winter;11(1-6):49-51. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<49::AID-JMR388>3.0.CO;2-3.
A fragment of the human low-density lipoprotein receptor encompassing the seven ligand-binding repeats fused to a C-terminal oligo-His tag was expressed in Sf9 insect cells. The melittin signal sequence encoded in the baculovirus vector led to secretion of the protein into the cell supernatant in a soluble form. The receptor fragment bound its natural ligand beta-migrating very-low-density lipoprotein and human rhinovirus serotype 2 in non-reducing ligand blots. Infection of all minor group human rhinovirus serotypes investigated was inhibited by the presence of the receptor fragment during viral challenge of HeLa cells. Infection is inhibited by aggregation of the virions.
一段包含七个配体结合重复序列并与C端寡聚组氨酸标签融合的人低密度脂蛋白受体片段在Sf9昆虫细胞中表达。杆状病毒载体中编码的蜂毒素信号序列使该蛋白以可溶形式分泌到细胞上清液中。在非还原配体印迹中，该受体片段可结合其天然配体β-迁移极低密度脂蛋白和人鼻病毒2型。在HeLa细胞受到病毒攻击期间，受体片段的存在可抑制所研究的所有小群人鼻病毒血清型的感染。病毒粒子的聚集可抑制感染。
