Bunin K V, Firsanov V I, Kravtsov E G, Sukhoroslova L I, Kapuka L P
Zh Mikrobiol Epidemiol Immunobiol. 1976 Oct(10):65-9.
Avidity of Vi-antibodies was studied in the patients suffering from typhoid fever and chronic carriers in the indirect hemagglutination test (IHAT) by means of titration with erythrocytic diagnostic agents sensitized with various doses of the Vi-antigen. The minimal sensitizing dose still adequate for detection of the antibodies in the titre of 1 : 20 was accepted as the avidity index. By the avidity sign the antibody heterogeneity index was calculated as the ratio of the optimal sensitizing dose to the minimal sensitizing one. In comparison with the patients suffering from typhoid fever of at the acute period of the disease in chronic carriers Vi-antibodies were more avid, this being conditioned chiefly by the highly avid IgG-antibodies. During the acute period of the disease the avidity of Vi-antibodies elevated only against the antibodies of the IgG-class. An increase of heterogeneity by the same sign was noted with increase of the degree of the serum antibody avidity.
通过用不同剂量Vi抗原致敏的红细胞诊断剂进行滴定，在间接血凝试验（IHAT）中研究了伤寒热患者和慢性携带者体内Vi抗体的亲和力。将仍足以检测到效价为1:20的抗体的最小致敏剂量作为亲和力指数。根据亲和力标志，将抗体异质性指数计算为最佳致敏剂量与最小致敏剂量之比。与疾病急性期的伤寒热患者相比，慢性携带者体内的Vi抗体亲和力更高，这主要由高亲和力的IgG抗体所致。在疾病急性期，Vi抗体的亲和力仅针对IgG类抗体升高。随着血清抗体亲和力程度的增加，观察到同一标志下异质性增加。
