使用戊二醛固定红细胞改进伤寒杆菌Vi抗体间接血凝试验。
Improvement of the indirect hemagglutination assay for Salmonella typhi Vi antibodies by use of glutaraldehyde-fixed erythrocytes.
作者信息
Barrett T J
出版信息
J Clin Microbiol. 1985 Oct;22(4):662-3. doi: 10.1128/jcm.22.4.662-663.1985.
Abstract
Glutaraldehyde-fixed sheep erythrocytes sensitized with Vi antigen were shown to be usable for at least 1 year in the indirect hemagglutination assay for Vi antibodies. The use of fixed sensitized erythrocytes improves the practicality of the indirect hemagglutination assay by (i) eliminating the need to sensitize cells each time the test is performed; (ii) reducing waste of the purified Vi antigen; and (iii) reducing test-to-test variation.
摘要
用Vi抗原致敏的戊二醛固定羊红细胞在Vi抗体间接血凝试验中至少可使用1年。使用固定的致敏红细胞可通过以下方式提高间接血凝试验的实用性：(i)每次进行试验时无需再致敏细胞；(ii)减少纯化Vi抗原的浪费；(iii)减少试验间的差异。
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Improvement of the indirect hemagglutination assay for Salmonella typhi Vi antibodies by use of glutaraldehyde-fixed erythrocytes.使用戊二醛固定红细胞改进伤寒杆菌Vi抗体间接血凝试验。
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