Jansson A, Goldstein M, Tinner B, Zoli M, Meador-Woodruff J H, Lew J Y, Levey A I, Watson S, Agnati L F, Fuxe K
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Neuroscience. 1999 Mar;89(2):473-89. doi: 10.1016/s0306-4522(98)00317-0.
The distribution of dopamine D1 and D2 receptor immunoreactivities in the nucleus accumbens and the olfactory tubercle of adult and postnatal male rats were compared with the distribution of tyrosine hydroxylase and dopamine transporter immunoreactivities. An overall co-distribution of D1 and D2 receptor immunoreactivities with tyrosine hydroxylase immunoreactivity was found in the nucleus accumbens and the olfactory tubercle. However, the major finding in this study was, following a more detailed analysis in coronal sections of the shell part of the nucleus accumbens, the existence of nerve cell patches of strong D1 receptor immunoreactivity associated with low D2 receptor, dopamine transporter and tyrosine hydroxylase immunoreactivities. These patches were mainly surrounded by areas of strong D2 receptor, tyrosine hydroxylase and dopamine transporter immunoreactivities and could be found also in the olfactory tubercle. Similar observations were made in postnatal rats. Serial reconstructions of the patches of strong D1 receptor immunoreactivity in the rostrocaudal direction were made. The patches formed a continuous tubular nerve cell system in the shell part of the nucleus accumbens. Since this nerve cell system was found to be surrounded by a high density of dopamine terminals, it may represent a compartment where dopamine transmission mainly acts on D1 receptors via local diffusion (i.e. via volume transmission). However, it must be noted that the D1 receptor rich patches constitute only a small fraction of the nucleus accumbens and the overall density of tyrosine hydroxylase immunoreactive terminals correlates with the density of both D1 and D2 receptors in the nucleus accumbens. In conclusion, the present paper gives new aspects on the chemical microarchitecture of the nucleus accumbens.
将成年和新生雄性大鼠伏隔核及嗅结节中多巴胺D1和D2受体免疫反应性的分布与酪氨酸羟化酶和多巴胺转运体免疫反应性的分布进行了比较。在伏隔核和嗅结节中发现D1和D2受体免疫反应性与酪氨酸羟化酶免疫反应性总体上共同分布。然而，本研究的主要发现是，在对伏隔核壳部冠状切片进行更详细分析后，存在强D1受体免疫反应性的神经细胞斑块，其与低D2受体、多巴胺转运体和酪氨酸羟化酶免疫反应性相关。这些斑块主要被强D2受体、酪氨酸羟化酶和多巴胺转运体免疫反应性区域所包围，在嗅结节中也能发现。在新生大鼠中也有类似的观察结果。对强D1受体免疫反应性斑块在前后方向进行了连续重建。这些斑块在伏隔核壳部形成了一个连续的管状神经细胞系统。由于发现这个神经细胞系统被高密度的多巴胺终末所包围，它可能代表一个多巴胺传递主要通过局部扩散（即通过容积传递）作用于D1受体的区室。然而，必须指出的是，富含D1受体的斑块仅占伏隔核的一小部分，酪氨酸羟化酶免疫反应性终末的总体密度与伏隔核中D1和D2受体的密度相关。总之，本文为伏隔核的化学微结构提供了新的视角。
