Hornstra G, Vergroesen A J
Acta Biol Med Ger. 1976;35(8-9):1065-8.
The effects of dietary linoleic acid on arterial thrombus formation in rats were compared with the inhibitory effect of intravenous or intraaortic administration of PGE1, a potent inhibitor of platelet adhesion and aggregation. The "rat aorta-loop" model proved to be a useful method to induce a stable thrombus, obstructing the aortae of standard-fed rats with an obstruction time (OT) of about 96 h. Increasing the amount of dietary linoleic acid from 2.5 cal% to 30 cal% doubled OT to about 200 h. A constant intravenous PGE1 infusion of 10 mug/h increased OT significantly from the control value of 126.6 h to 160.1 h. When the same amount of PGE1 was infused intraarteriallu, the increase in OT was higher and doubled to 255.7 h. Using the "Filtragometer method" - in which platelet aggregatibility is measured in flowing human venous blood - a significant decrease in platelet stickiness was found in a group of patients consuming 12 cal% linoleic acid as compared to a similar group of patients with only 4 cal% linoleic acid in the diet. As similar results were obtained in patients with diabetes mellitus and in survivors of a myocardial infarction, increasing the amount of dietary linoleic acid may be of great therapeutic value for patients at risk of arterial thrombotic processes. These data support the hypothesis that part of the excess of dietary linoleic acid can lead to increased PGE1 concentrations.
将膳食亚油酸对大鼠动脉血栓形成的影响与静脉内或主动脉内给予PGE1（一种血小板黏附和聚集的强效抑制剂）的抑制作用进行了比较。“大鼠主动脉环”模型被证明是诱导稳定血栓的一种有用方法，该模型使标准饮食大鼠的主动脉阻塞，阻塞时间（OT）约为96小时。将膳食亚油酸的量从2.5卡路里%增加到30卡路里%，使OT增加了一倍，达到约200小时。以10微克/小时的恒定速率静脉内输注PGE1，使OT从对照值126.6小时显著增加到160.1小时。当以相同量的PGE1进行动脉内输注时，OT的增加更高，增加了一倍，达到255.7小时。使用“滤过计法”（即在流动的人体静脉血中测量血小板聚集性），发现一组摄入12卡路里%亚油酸的患者与饮食中仅含4卡路里%亚油酸的类似患者组相比，血小板黏附性显著降低。由于在糖尿病患者和心肌梗死幸存者中也获得了类似结果，增加膳食亚油酸的量可能对有动脉血栓形成风险的患者具有很大的治疗价值。这些数据支持这样的假设，即膳食亚油酸过量的一部分可导致PGE1浓度升高。
