Elmadfa I, Grünberg P, Schlotzer E
Int J Vitam Nutr Res. 1983;53(2):210-7.
In order to estimate the linoleic acid requirement of the rat, four groups of weanling male Wistar-Rats, 18 animals each, were fed isoenergetic semi-synthetic diets containing 14 cal% fat. The linoleic acid content (as linoleic acid methyl ester) amounted to 0; 0.5; 1.3 and 4.0% of total energy intake. The experiment lasted 14 weeks. The parameters analysed were the concentrations of total lipids (TL), free cholesterol (Ch), cholesterol esters (ChE), triglycerides (TG) and phospholipids (PL) in plasma and liver. - Clinical signs of linoleic acid deficiency were only found in the rats fed the linoleic acid free diet. With 0.5 cal% linoleic acid in the diet no deficiency symptoms were observed. - In plasma of the linoleic acid deficient animals the concentrations of TL, Ch, ChE and TG were decreased. Plasma PL contents were insignificantly altered. - While the contents of TL, TG and ChE in the liver of the deficient rats increased significantly, those of PL and Ch were hardly affected. The results show that a linoleic acid supply of 0.5 cal% prevents nearly all alterations of plasma and liver lipid concentrations noticed in linoleic acid deficiency. The effect of this dose was as good as that of 1.3 cal% linoleic acid. Therefore we assume that the minimum requirement of male young rats for linoleic acid is markedly less than 1,3% of total energy intake.
为了评估大鼠对亚油酸的需求量，将四组断乳雄性Wistar大鼠，每组18只，喂食含14%热量脂肪的等能量半合成日粮。亚油酸含量（以亚油酸甲酯计）占总能量摄入量的0%、0.5%、1.3%和4.0%。实验持续14周。分析的参数包括血浆和肝脏中总脂质（TL）、游离胆固醇（Ch）、胆固醇酯（ChE）、甘油三酯（TG）和磷脂（PL）的浓度。——仅在喂食无亚油酸日粮的大鼠中发现了亚油酸缺乏的临床症状。日粮中亚油酸含量为0.5%热量时，未观察到缺乏症状。——在亚油酸缺乏动物的血浆中，TL、Ch、ChE和TG的浓度降低。血浆PL含量变化不显著。——虽然缺乏亚油酸的大鼠肝脏中TL、TG和ChE的含量显著增加，但PL和Ch的含量几乎未受影响。结果表明，0.5%热量的亚油酸供应可防止亚油酸缺乏时血浆和肝脏脂质浓度出现的几乎所有变化。该剂量的效果与1.3%热量的亚油酸效果相同。因此，我们认为雄性幼鼠对亚油酸的最低需求量明显低于总能量摄入量的1.3%。
