RSC，一种必需的、丰富的染色质重塑复合体。

RSC, an essential, abundant chromatin-remodeling complex.

作者信息

Cairns B R, Lorch Y, Li Y, Zhang M, Lacomis L, Erdjument-Bromage H, Tempst P, Du J, Laurent B, Kornberg R D

机构信息

Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.

出版信息

Cell. 1996 Dec 27;87(7):1249-60. doi: 10.1016/s0092-8674(00)81820-6.

DOI:10.1016/s0092-8674(00)81820-6

PMID:8980231

Abstract

A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWII/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.

摘要

一种具有重塑染色质结构能力的新型15亚基复合物，称为RSC，已基于与SWI/SNF复合物的同源性从酿酒酵母中分离出来。至少有三个RSC亚基与SWI/SNF多肽相关：Sth1p、Rsc6p和Rsc8p分别与Swi2/Snf2p、Swp73p和Swi3p显著相似，并通过肽片段的质谱分析和序列分析得以鉴定。与SWI/SNF一样，RSC表现出由游离DNA和核小体DNA刺激的DNA依赖性ATP酶活性以及扰乱核小体结构的能力。然而，RSC的丰度比SWI/SNF复合物至少高10倍，并且对有丝分裂生长至关重要。与关于SWII/SNF复合物的一份报告相反，未检测到RSC（或SWI/SNF复合物）与RNA聚合酶II全酶的关联。