Sinitsyn V V, Bokchubaev E T, Mamontova A G, Ovtrakht N V, Nasonov E L, Konovalov G A, Kukharchuk V V
Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow, Russia.
Artif Organs. 1992 Jun;16(3):291-3. doi: 10.1111/j.1525-1594.1992.tb00312.x.
Plasmasorption on a heparin-based sorbent was performed in vitro. It demonstrated affinity of the C3a and C5a anaphylatoxins for the sorbent: C3a was removed almost completely (97%), and the C5a concentration decreased on average by 55%. The plasma level of C3a and C5a complement components was also monitored during the procedure of clinical extracorporeal low density lipoprotein (LDL) apheresis on the sorbent in patients with familial hypercholesterolemia. A two- to threefold increase in C3a (up to 1,500 ng/ml) was observed after plasma separation by the IBM 2997 cell sorter. Subsequent processing of the plasma through the column led to the low level of C3a detected (less than 50 ng/ml), demonstrating significant uptake of C3a by the sorbent column. The removed C3a was found in the eluate obtained after regeneration of the sorbent with 2 M NaCl solution. No significant increase in C5a was found during the procedure. Nevertheless, some C5a was detected in the eluate from the sorbent. The content of C3a and C5a in patients blood after the treatment was approximately the same as it was initially, 200-500 ng/ml for C3a and less than 10 ng/ml for C5a. The removal of C3a and C5a anaphylatoxins by heparin-based sorbent should be regarded as an advantage of this type of plasmasorbent.
在体外进行了基于肝素的吸附剂上的血浆吸附实验。实验证明了过敏毒素C3a和C5a对该吸附剂具有亲和力：C3a几乎被完全去除（97%），C5a浓度平均下降了55%。在家族性高胆固醇血症患者使用该吸附剂进行临床体外低密度脂蛋白（LDL）血液成分分离术的过程中，也监测了C3a和C5a补体成分的血浆水平。在用IBM 2997细胞分选仪进行血浆分离后，观察到C3a增加了两到三倍（高达1500 ng/ml）。随后血浆通过柱子处理后，检测到C3a水平较低（低于50 ng/ml），这表明吸附剂柱对C3a有显著的摄取作用。在用2 M NaCl溶液对吸附剂进行再生后得到的洗脱液中发现了被去除的C3a。在该过程中未发现C5a有显著增加。然而，在吸附剂的洗脱液中检测到了一些C5a。治疗后患者血液中C3a和C5a的含量与初始时大致相同，C3a为200 - 500 ng/ml，C5a低于10 ng/ml。基于肝素的吸附剂去除C3a和C5a过敏毒素应被视为这种类型血浆吸附剂的一个优点。
