Tridon A, Palcoux J B, Jouanel P, Bezou M J, Coulet M, Betail G
Laboratoire d'Immunologie, CHRU Clermont-Ferrand, France.
Artif Organs. 1992 Dec;16(6):577-85. doi: 10.1111/j.1525-1594.1992.tb00555.x.
Complement system activation was investigated in two girls with familial homozygous hypercholesterolemia undergoing two monthly sessions on LA15 or LA40 (Kaneka liposorber). We determined blood levels of C3c and C3a, leukocyte counts, and plasma levels of C3c and C3a in the extracorporeal circulation device at the start of the sessions and 15 and either 60 or 120 min into them. Sequential eluates were collected from LA40 at the end of the sessions (0.5M NaCl, 1M hydroxylamine). Anaphylatoxin C3a increased throughout, especially with LA40. As previously reported, C3a was trapped in the dextran column but was noticeably present in efferent plasma. Besides many proteins, nonnative complement fragments bearing C3a and C3d antigens were detected in almost all the eluates, suggesting possible in situ complement activation. Practically, complement activation induced by the first filter is a risk; long-term side effects may arise from this extracorporeal circulation device.
对两名患有家族性纯合子高胆固醇血症的女孩进行了补体系统激活情况的研究，她们每月接受两次关于LA15或LA40（Kaneka脂质吸附器）的治疗。我们测定了治疗开始时、治疗15分钟以及60或120分钟时体外循环装置中C3c和C3a的血药浓度、白细胞计数以及血浆中C3c和C3a的水平。在治疗结束时从LA40收集连续洗脱液（0.5M氯化钠、1M羟胺）。过敏毒素C3a在整个过程中均升高，尤其是使用LA40时。如先前报道，C3a被困在葡聚糖柱中，但在流出血浆中明显存在。除了许多蛋白质外，在几乎所有洗脱液中都检测到了带有C3a和C3d抗原的非天然补体片段，这表明可能存在原位补体激活。实际上，第一个滤器诱导的补体激活是一种风险；这种体外循环装置可能会产生长期副作用。
