Amundson D M, Zhou M
Molecular Probes, Inc., Eugene, OR 97402, USA.
J Biochem Biophys Methods. 1999 Jan 13;38(1):43-52. doi: 10.1016/s0165-022x(98)00036-0.
A fluorometric method for the enzymatic determination of cholesterol content has been developed using a novel fluorogenic H2O2 probe, Amplex Red. This assay is performed in a 96-well microplate, and it is a one-step method amenable to automated procedures. Using commercially available cholesterol, our assay allows detection of 5 pmol (2 ng) cholesterol per well, which is 100-fold more sensitive than published fluorometric and colorimetric methods. When applied to the measurement of cholesterol levels in serum and food samples, the Amplex Red-based method has been found more attractive since the oxidation product of the Amplex Red method has superior long wavelength spectra which are less susceptible to interference from the biological compounds.
已开发出一种使用新型荧光H2O2探针Amplex Red进行酶法测定胆固醇含量的荧光测定方法。该测定在96孔微孔板中进行，是一种适用于自动化程序的一步法。使用市售胆固醇，我们的测定法可检测到每孔5皮摩尔（2纳克）胆固醇，比已发表的荧光法和比色法灵敏100倍。当应用于血清和食品样品中胆固醇水平的测量时，基于Amplex Red的方法更具吸引力，因为Amplex Red方法的氧化产物具有优异的长波长光谱，不易受到生物化合物的干扰。
