对于精液分析正常但仓鼠卵穿透试验失败的接受体外受精-胚胎移植的夫妇，胞浆内单精子注射有必要吗？
Is intracytoplasmic sperm injection necessary for couples undergoing in vitro fertilization-embryo transfer with normal semen analyses but failing hamster egg penetration assays?
作者信息
Surrey E S, Lee G S, Surrey M W, Hill D
机构信息
Reproductive Medicine and Surgery Associates, Beverly Hills, California, USA.
出版信息
J Assist Reprod Genet. 1999 Feb;16(2):69-72. doi: 10.1023/a:1022512705515.
PURPOSE
Our purpose was to assess whether in vitro fertilization (IVF)-embryo transfer (ET) candidate couples with basically normal semen analyses but failing zona-free hamster egg penetration assay (HEPA) scores benefit from intracytoplasmic sperm injection (ICSI).
METHODS
Twenty consecutive IVF candidate couples with normal-borderline semen analyses and failing HEPA scores were recruited. Mature oocytes obtained from each woman were randomly divided between ICSI (group I; n = 126 oocytes) and standard insemination techniques (group II; 138 oocytes). Fertilization (two pronuclei) and cleavage (2-4 cells) rates were assessed for both groups.
RESULTS
There were no statistically significant differences between the two groups with respect to (mean +/- standard error of the mean) fertilization (group I, 63.1 +/- 7.75; group II, 77.8 +/- 4.7%) or cleavage (group I, 87.3 +/- 2.4%; group II, 91.2 +/- 3.5%) rates.
CONCLUSIONS
ICSI is not beneficial for IVF-ET when sperm samples demonstrate a failing HEPA score but have normal or minimally compromised semen analysis parameters.
目的
我们的目的是评估精液分析基本正常但无透明带仓鼠卵穿透试验（HEPA）评分失败的体外受精（IVF）-胚胎移植（ET）候选夫妇是否能从卵胞浆内单精子注射（ICSI）中获益。
方法
招募了20对连续的IVF候选夫妇，他们的精液分析处于正常临界值且HEPA评分失败。从每位女性获得的成熟卵母细胞被随机分为ICSI组（第I组；126个卵母细胞）和标准授精技术组（第II组；138个卵母细胞）。评估两组的受精（两个原核）和卵裂（2-4细胞）率。
结果
两组在（平均±平均标准误）受精率（第I组，63.1±7.75；第II组，77.8±4.7%）或卵裂率（第I组，87.3±2.4%；第II组，91.2±3.5%）方面没有统计学上的显著差异。
结论
当精子样本HEPA评分失败但精液分析参数正常或仅有轻微受损时，ICSI对IVF-ET并无益处。
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