Aitken R J, Best F S, Warner P, Templeton A
J Androl. 1984 Jul-Aug;5(4):297-303. doi: 10.1002/j.1939-4640.1984.tb00792.x.
The male partners of 68 couples exhibiting 5.1 +/- 0.3 (SEM) years of unexplained infertility were assessed using the conventional criteria of semen quality, the movement characteristics of the spermatozoa and the outcome of the zona-free hamster egg penetration test. After a follow-up period of 2.3 +/- 0.06 (SEM) years, 25 (37%) of these patients were found to have initiated a pregnancy, thereby permitting an analysis of those aspects of semen quality which most accurately predicted their subsequent fertility. A multivariate discriminant analysis revealed that the conventional semen profile, per se, was not of significant value in discriminating the incidence of pregnancy. However, significant discrimination (P = 0.0173) was obtained when the postcapacitation movement characteristics of the spermatozoa were incorporated into the analysis. The accuracy of this prognosis was further increased if either the duration of infertility or the outcome of the zona-free hamster egg penetration test was taken into consideration. Overall classifications of fertility were then 76.3% and 76.5% accurate, respectively. These results suggest that in vitro assessments of human sperm function are of significant value in evaluating male fertility.
采用精液质量的传统标准、精子运动特征以及无透明带仓鼠卵穿透试验结果，对68对患有5.1±0.3（标准误）年不明原因不孕症夫妇的男性伴侣进行了评估。经过2.3±0.06（标准误）年的随访期后，发现其中25名（37%）患者已开始妊娠，从而能够对精液质量中最准确预测其后续生育能力的那些方面进行分析。多变量判别分析显示，传统的精液概况本身在区分妊娠发生率方面并无显著价值。然而，当将精子获能后的运动特征纳入分析时，得到了显著的区分效果（P = 0.0173）。如果考虑不孕持续时间或无透明带仓鼠卵穿透试验结果，这种预后的准确性会进一步提高。总体生育能力分类的准确率分别为76.3%和76.5%。这些结果表明，体外评估人类精子功能在评估男性生育能力方面具有重要价值。
