College of Food and Bioengineering, Henan University of Science and Technology, No. 70, Tianjing Road, 471003 Luoyang, Henan, People's Republic of China.
Colloids Surf B Biointerfaces. 2010 Jul 1;78(2):367-71. doi: 10.1016/j.colsurfb.2010.02.028. Epub 2010 Mar 4.
Audible sound, one of the environmental factors, widely exists in natural world. However, the interaction between audible sound and biological materials is usually neglected in the field of biological research. Very little efforts have been put forth in studying the relation of organisms and audible sound. Here we investigated the response of Escherichia coli cells to the stimulation by audible sound under the normal condition and environmental stresses. The results showed that the audible sound treatment significantly increases the colony forming of E. coli under the normal growth condition. However, under osmotic stress induced by the sugar, audible sound stimulation may enhance the inhibitory effect of osmotic stress on E. coli growth. More interestingly, audible sound treatment seems to alleviate the inhibitory effect of salt stress on E. coli growth when the concentration of sodium chloride was increased to 30 g/l, although the action of sound waves of audible frequency is likely to evoke an inhibition of the growth of E. coli in the medium containing 20 g/l of sodium chloride. Some potential mechanisms may be involved in the responses of bacterial cells to audible sound stimulation.
可听声，作为环境因素之一，广泛存在于自然界中。然而，在生物研究领域，通常会忽视可听声与生物材料之间的相互作用。在研究生物体与可听声的关系方面，人们付出的努力微乎其微。在这里，我们研究了在正常条件和环境胁迫下大肠杆菌细胞对可听声刺激的反应。结果表明，在正常生长条件下，可听声处理可显著增加大肠杆菌的菌落形成。然而，在糖诱导的渗透压胁迫下，可听声刺激可能会增强渗透压胁迫对大肠杆菌生长的抑制作用。更有趣的是，当氯化钠浓度增加到 30g/L 时，可听声处理似乎减轻了盐胁迫对大肠杆菌生长的抑制作用，尽管在含有 20g/L 氯化钠的培养基中，可听声的声波作用很可能会抑制大肠杆菌的生长。细菌细胞对可听声刺激的反应可能涉及一些潜在的机制。
