钠钾ATP酶的抗氧化性
Oxidative resistance of Na/K-ATPase.
作者信息
Kurella E G, Tyulina O V, Boldyrev A A
机构信息
Laboratory of Clinical Neurochemistry, Institute of Neurology, Russian Academy of Medical Sciences, Moscow, Russia.
出版信息
Cell Mol Neurobiol. 1999 Feb;19(1):133-40. doi: 10.1023/a:1006976810642.
Abstract
- Oxidative modification of Na/K-ATPase from brain and kidney has been studied. Brain enzyme has been found to be more sensitive than kidney enzyme to inhibition by both H2O2 and NaOCl. 2. The inhibition of Na/K-ATPase correlates well with the decrease in a number of SH groups, suggesting that the latter belong mainly to ATPase protein and are essential for the enzyme activity. We suggest that the differences in the number, location, and accessibility of SH groups in Na/K-ATPase isozymes predict their oxidative stability. 3. The hydrophilic natural antioxidant carnosine, the hydrophobic natural antioxidant alpha-tocopherol, and the synthetic antioxidant ionol as well as the ferrous ion chelating agent deferoxamine were found to protect Na/K-ATPase from oxidation by different concentrations of H2O2. The data suggest that these antioxidants are effective due to their ability to neutralize or to prevent formation of hydroxyl radicals.
摘要
- 已对来自脑和肾的钠钾ATP酶的氧化修饰进行了研究。发现脑酶比肾酶对过氧化氢和次氯酸钠的抑制更敏感。2. 钠钾ATP酶的抑制与巯基数量的减少密切相关，这表明后者主要属于ATP酶蛋白，并且对酶活性至关重要。我们认为钠钾ATP酶同工酶中巯基数量、位置和可及性的差异预示了它们的氧化稳定性。3. 发现亲水性天然抗氧化剂肌肽、疏水性天然抗氧化剂α-生育酚、合成抗氧化剂异壬醇以及亚铁离子螯合剂去铁胺可保护钠钾ATP酶免受不同浓度过氧化氢的氧化。数据表明这些抗氧化剂有效是因为它们具有中和或防止羟基自由基形成的能力。
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