肌肽及相关化合物对活性氧生成的影响：一项比较研究。

Effects of carnosine and related compounds on generation of free oxygen species: a comparative study.

作者信息

Boldyrev A, Abe H, Stvolinsky S, Tyulina O

机构信息

Department of Biochemistry, Moscow State University, Russia.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 1995 Nov;112(3):481-5. doi: 10.1016/0305-0491(95)00084-4.

DOI:10.1016/0305-0491(95)00084-4

PMID:8529024

Abstract

The effect of carnosine and related compounds on the luminol- and lucigenin-dependent luminescence of rabbit leukocytes, activated by BaSO4, has been studied. Carnosine was found to modify BaSO4-induced chemiluminescence of leukocytes via suppression of hypochlorous anion generation with simultaneous stimulation the system of oxygen superoxide anion. Additionally to this effect carnosine prevents enzymic dismutation of O2.. Anserine, acetylanserine, and homocarnosine also possess the ability to activate O.2 production by leukocytes. The effect is not inherent to imidazole used in the same concentrations. Suppression of myeloperoxidase reaction by carnosine and related compounds is explained by both inhibiting action on the enzyme itself, and direct neutralization of hypochlorous anion due to formation of stable chloroamine complexes. Methylation of carnosine at N1 nitrogen of imidazole ring, leading to anserine, forced inhibition of myeloperoxidase system, whereas its acetylation at the free beta-amino group weakened this effect.

摘要

已研究了肌肽及相关化合物对经硫酸钡激活的兔白细胞中鲁米诺和光泽精依赖性发光的影响。发现肌肽可通过抑制次氯酸根阴离子的产生，同时刺激超氧阴离子系统，来改变硫酸钡诱导的白细胞化学发光。除了这种作用外，肌肽还可防止O₂⁻的酶促歧化反应。鹅肌肽、乙酰鹅肌肽和高肌肽也具有激活白细胞产生O₂⁻的能力。相同浓度的咪唑则没有这种作用。肌肽及相关化合物对髓过氧化物酶反应的抑制作用，既归因于对该酶本身的抑制作用，也归因于由于形成稳定的氯胺络合物而对次氯酸根阴离子的直接中和作用。肌肽咪唑环N1氮原子甲基化生成鹅肌肽，会增强对髓过氧化物酶系统的抑制作用，而其游离β-氨基乙酰化则会减弱这种作用。