Vernet-Nyssen M
Ann Biol Clin (Paris). 1976;34(4):259-68.
After recalling the conditions of fixation of iron on human native serum, the authors determined the TIBC on 15 commercial control sera after titration. The transferrin concentration was determined on 13 sera of human origin, by two immunochemical techniques. They were thus able to demonstrate certain sera including a chelator substance other than transferrin, their TIBC depends only on the experimental conditions; such sera are not usable for quality control. Lyophilisation seems to reduce the reactivity of sites of fixation. It is thus necessary to provide a large excess of iron and prolong the duration of contact.
在回顾了铁在人天然血清中的固定条件后，作者在滴定后测定了15种商业对照血清的总铁结合力（TIBC）。通过两种免疫化学技术测定了13份人源血清中的转铁蛋白浓度。他们因此能够证明某些血清中除了转铁蛋白外还含有一种螯合剂物质，其TIBC仅取决于实验条件；这类血清不可用于质量控制。冻干似乎会降低固定位点的反应性。因此有必要提供大量过量的铁并延长接触时间。
