Laurent P
Rev Can Biol. 1978 Jun;37(2):127-30.
The "thermostable" B. thuringiensis exotoxin is active on cell cultures of Mammals "in vitro", except on the KB strain from a human tumor. The primary cultures are the most sensitive: first, with monkey kidney cells, the growth is inhibited by 0.1 mg of toxin per ml; next, the young rabbit kidney cells react to 0.25 mg of toxin per ml. The established lines of cells come last: human diploid cells (Lyon 4) and heteroploid cells (BHK21C13), with the same active dose of 1 mg of toxin per ml. No protection is obtained by adding ATP to monkey kidney cells at the same time as the exotoxin.
“热稳定”的苏云金芽孢杆菌外毒素在体外对哺乳动物细胞培养物有活性,但对源自人类肿瘤的KB细胞株无活性。原代培养物最为敏感:首先,对于猴肾细胞,每毫升0.1毫克毒素可抑制其生长;其次,幼兔肾细胞对每毫升0.25毫克毒素有反应。细胞系的敏感性最低:人二倍体细胞(Lyon 4)和异倍体细胞(BHK21C13),相同的活性剂量为每毫升1毫克毒素。在外毒素作用于猴肾细胞的同时添加ATP并不能提供保护作用。