Avent M L, Gal P, Ransom J L, Brown Y L, Hansen C J, Ricketts W A, Soza F
Greensboro Area Health Education Center, Moses Cone Health System, NC, USA.
Ann Pharmacother. 1999 Feb;33(2):144-8. doi: 10.1345/aph.17426.
To evaluate drug delivery to the lungs of nebulized and metered-dose inhalers (MDIs) in an in vitro infant lung model.
An in vitro lung model was modified to study drug delivery. A 1000 mL intravenous bag filled with 500 mL deionized water was attached to a 3.5 mm (12 cm length) endotracheal tube. An inline Marquest Whisper Jet infant circuit nebulizer system delivered 2.5 mg/3 mL albuterol sulfate inhalation solution (Ventolin nebules) at a flow rate of 5 L/min. An Aerochamber (Monaghan) was placed at the endotracheal tube for the delivery of the MDIs. Albuterol MDI (Ventolin) 10 inhalations and beclomethasone MDI (Beclovent) 20 inhalations were delivered. A Servo 900C (Siemens-Elma) was used at the following ventilator settings: positive inspiratory pressure 30 cm H2O), intermittent mandatory ventilation 40 breaths/min, positive end expiratory pressure 4 cm H2O, inspiratory time 0.4 sec. Each formulation was run at least 10 times and assayed in duplicate by HPLC. An unpaired Student's t-test was used to analyze the statistical significance of the data.
There was a significantly greater percentage of drug delivery with MDI albuterol (1.96 +/- 0.50) as compared with nebulized albuterol (1.26 +/- 0.37) (p = 0.002) or beclomethasone diproprionate (0.51 +/- 0.24) (p = 0.001).
Albuterol MDI provides a more efficient delivery of drug to the lung as compared with nebulized albuterol and MDI beclomethasone diproprionate.
在体外婴儿肺模型中评估雾化吸入器和定量吸入器（MDIs）对肺部的药物递送情况。
对体外肺模型进行改良以研究药物递送。一个装有500 mL去离子水的1000 mL静脉输液袋连接到一根3.5 mm（12 cm长）的气管导管上。一个在线的Marquest Whisper Jet婴儿回路雾化器系统以5 L/min的流速递送2.5 mg/3 mL硫酸沙丁胺醇吸入溶液（万托林雾化液）。在气管导管处放置一个储物罐（莫纳根公司）用于MDIs的递送。递送了沙丁胺醇MDI（万托林）10次吸入量和倍氯米松MDI（必可酮）20次吸入量。使用Servo 900C（西门子 - 埃尔马公司），设置以下通气参数：吸气正压30 cm H₂O，间歇强制通气40次/分钟，呼气末正压4 cm H₂O，吸气时间0.4秒。每种制剂至少运行10次，并通过高效液相色谱法进行双份测定。使用未配对的学生t检验分析数据的统计学意义。
与雾化沙丁胺醇（1.26 +/- 0.37）（p = 0.002）或二丙酸倍氯米松（0.51 +/- 0.24）（p = 0.001）相比，MDI沙丁胺醇的药物递送百分比显著更高（1.96 +/- 0.50）。
与雾化沙丁胺醇和MDI二丙酸倍氯米松相比，沙丁胺醇MDI向肺部递送药物的效率更高。
