Flesch I E, Kaufmann S H
Department of Immunology, University Clinics Ulm, Germany.
Immunobiology. 1999 Feb;200(1):120-7. doi: 10.1016/S0171-2985(99)80037-0.
Bone marrow-derived macrophages (BMM) comprise a population of quiescent cells which can be activated by defined signals. Here, we directly compare the release of chemokines and monokines by BMM raised either in serum-supplemented or in serum-free medium in response to Listeria monocytogenes EGD or Mycobacterium bovis BCG infection. We focused on this issue because there have been several controversial reports on the production of cytokines by BMM due to different in vitro culture conditions. Culture in serum-supplemented medium primed BMM for release of monocyte chemoattractant protein (MCP)-1, interleukin (IL)-6, and IL-12, but had no effect on macrophage inflammatory protein (MIP)-1alpha and tumor necrosis factor (TNF)-alpha production in response to L. monocytogenes infection. After challenge infection with M. bovis, BMM raised and stimulated in serum-supplemented medium secreted higher levels of MCP-1, MIP-1alpha, IL-6, and TNF-alpha but not of IL-12 as compared to BMM cultured and infected in a serum-free medium. The effects of serum could be partially mimicked by interferon-gamma. Because the serum components responsible for BMM priming are undefined, BMM cultured under serum-free conditions provide an appropriate cell population for defining macrophage activating signals.
骨髓来源的巨噬细胞（BMM）由一群静止细胞组成，这些细胞可被特定信号激活。在此，我们直接比较了在补充血清或无血清培养基中培养的BMM，在受到单核细胞增生李斯特菌EGD或牛分枝杆菌卡介苗感染时趋化因子和单核因子的释放情况。我们关注这个问题是因为由于不同的体外培养条件，关于BMM产生细胞因子的报道存在一些争议。在补充血清的培养基中培养使BMM对单核细胞趋化蛋白（MCP）-1、白细胞介素（IL）-6和IL-12的释放做好了准备，但对单核细胞趋化蛋白-1α和肿瘤坏死因子（TNF）-α在应对单核细胞增生李斯特菌感染时的产生没有影响。在用牛分枝杆菌进行激发感染后，与在无血清培养基中培养和感染的BMM相比，在补充血清的培养基中培养并受到刺激的BMM分泌更高水平的MCP-1、MIP-1α、IL-6和TNF-α，但不分泌IL-12。血清的作用可被γ干扰素部分模拟。由于负责BMM启动的血清成分尚不清楚，在无血清条件下培养的BMM为确定巨噬细胞激活信号提供了合适的细胞群体。
