Erler W, Schimmel D, Steinbach G
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Bereich Jena.
Berl Munch Tierarztl Wochenschr. 1997 Oct;110(10):365-8.
The relative contents of long-chain fatty acids in P. multocida and P. haemolytica were investigated. A dependence on the composition of the broth was established. Accordingly, comparative quantitative studies on fatty acid contents have to be conducted using bacteria grown with the same lot of broth medium. As for P. multocida, there were significant differences between the serovars (C14 in TDHM and C16, delta 2C18 in BPL). These differences are, however, not significant to replace serotyping. Highly significant differences were also detected between P. multocida isolates from nasal swabs and pneumonic lungs (interims of C14, delta C16 on BPL and BRU). The largest differences were measured for strains grown on BRU, which is interpreted as an expression of virulence. Significant differences were found between biotypes A and T of P. haemolytica, namely for C14, C16 in TDHM, and C14, delta C16, C16, C18 in BPL medium.
研究了多杀性巴氏杆菌和溶血巴氏杆菌中长链脂肪酸的相对含量。确定了其对肉汤成分的依赖性。因此,必须使用在同一批次肉汤培养基中培养的细菌进行脂肪酸含量的比较定量研究。至于多杀性巴氏杆菌,不同血清型之间存在显著差异(TDHM中的C14以及BPL中的C16、δ2C18)。然而,这些差异对于取代血清分型并不显著。在来自鼻拭子和肺炎肺组织的多杀性巴氏杆菌分离株之间也检测到高度显著差异(BPL和BRU中的C14、δC16方面)。在BRU上生长的菌株差异最大,这被解释为毒力的一种表现。溶血巴氏杆菌的生物型A和T之间存在显著差异,即在TDHM中的C14、C16以及BPL培养基中的C14、δC16、C16、C18方面。