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[犊牛鼻黏膜上产毒素非典型巴氏杆菌菌株的检测及其对溶血巴氏杆菌和多杀性巴氏杆菌的鉴别诊断界限]

[Detection of atypical toxin-producing Pasteurella strains on the nasal mucosa of calves and their differential diagnostic limits for Pasteurella haemolytica and Pasteurella multocida].

作者信息

Krüger M, Zucker B, Flegel T, Horst I, Grund S, Horsch F

机构信息

Institut für Mikrobiologie und Tierseuchen, Fachbereich Veterinärmedizin der Freien Universität Berlin.

出版信息

Berl Munch Tierarztl Wochenschr. 1994 Jan;107(1):15-9.

PMID:8129703
Abstract

The bacterial colonisation on nasal mucous membranes of calves at age of 3, 5 to 5 months was investigated by cotton swabs. Three Pasteurella species were found (P. multocida subspecies multocida, P. haemolytica, P. avium) as monocausal infection as well as pluricausal infection. P. avium was characterised by colonial morphology, bacterioscopy, biochemical properties, polypeptide-pattern in SDS-PAGE and by electronmicroscopic investigation and was differentiated from P. multocida and P. haemolytica. It is very interesting, that the P. avium strains possess an antigen structure, reacting with monoclonal antibodies directed against the heat labile-toxin of P. multocida subspecies multocida.

摘要

用棉拭子对3周龄、5至5月龄犊牛鼻黏膜上的细菌定植情况进行了调查。发现了三种巴氏杆菌(多杀性巴氏杆菌多杀亚种、溶血巴氏杆菌、鸟巴氏杆菌),它们既可以是单一病因感染,也可以是多种病因感染。通过菌落形态、细菌学检查、生化特性、SDS - PAGE中的多肽图谱以及电子显微镜检查对鸟巴氏杆菌进行了鉴定,并将其与多杀性巴氏杆菌和溶血巴氏杆菌区分开来。非常有趣的是,鸟巴氏杆菌菌株具有一种抗原结构,能与针对多杀性巴氏杆菌多杀亚种不耐热毒素的单克隆抗体发生反应。

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