Blanco-Viera F J, Trigo F J, Jaramillo-Meza L, Aguilar-Romero F
Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, D.F., Mexico.
Rev Latinoam Microbiol. 1995 Apr-Jun;37(2):121-6.
A total of 13,000 pairs of lungs were examined at Mexico's City abbatoir, where 8,000 corresponded to sheep and 5,000 to cattle. From those, 224 pneumonic lesions were observed, obtaining 97 positive isolates, which yielded 112 strains of Pasteurella sp. Forty isolates were identified as P. haemolytica and 72 as P. multocida. One-hundred percent of P. haemolytica belonged to biotype A. Serotypes were determined by indirect haemagglutination. P. multocida isolates were classified according to the acriflavine and hyaluronidase techniques, 61% belonged to type A, 25% to type D and 14% were untypified. Somatic serotypes were determined by gel immunodiffusion; serotype 3 was more frequent, in sheep 72% and in cattle 77%.
在墨西哥城的屠宰场共检查了13000对肺脏,其中8000对来自绵羊,5000对来自牛。从中观察到224处肺炎病变,获得97株阳性分离株,从中分离出112株巴氏杆菌属菌株。鉴定出40株溶血巴氏杆菌和72株多杀巴氏杆菌。100%的溶血巴氏杆菌属于生物型A。通过间接血凝试验确定血清型。多杀巴氏杆菌分离株根据吖啶黄和透明质酸酶技术进行分类,61%属于A型,25%属于D型,14%未分型。通过凝胶免疫扩散确定菌体血清型;血清型3最为常见,在绵羊中占72%,在牛中占77%。