Liszka M, Zukowska-Szczechowska E, Grzeszczak W, Moczulski D, Religa Z
Katedra i Klinika Chorób Wewnetrznych i Diabetologii w Zabrzu.
Pol Arch Med Wewn. 1998 Jul;100(1):9-18.
The patients with chronic renal failure present an immunodeficiency state manifested by prolonged tolerance to allografts, increased incidence of infections and abnormally high incidence of neoplasia. The present study aimed to assess the effect of chronic uraemia and haemodialysis treatment on the natural killer cells (NK cells) count. Peripheral blood NK cells (CD3-, CD16+), total lymphocytes, leukocytes, monocytes and granulocytes of 24 hemodialyzed patients with chronic renal failure and 32 healthy subjects were studied using flow cytometry. In the investigated group of patients with chronic renal failure treated with haemodialysis the count of NK cells (CD3-, CD16+) in the peripheral blood was significantly decreased in comparison to healthy subjects (137 +/- 11 versus 229 +/- 13, p < 0.001) and a significant negative correlation (r = -0.391, p < 0.05) was observed between the duration of haemodialysis treatment and the count of NK cells (CD3-, CD16+).
慢性肾衰竭患者呈现免疫缺陷状态，表现为对同种异体移植物的耐受性延长、感染发生率增加以及肿瘤发生率异常升高。本研究旨在评估慢性尿毒症和血液透析治疗对自然杀伤细胞（NK细胞）计数的影响。使用流式细胞术对24例接受血液透析的慢性肾衰竭患者和32例健康受试者的外周血NK细胞（CD3⁻、CD16⁺）、总淋巴细胞、白细胞、单核细胞和粒细胞进行了研究。在接受血液透析治疗的慢性肾衰竭患者研究组中，外周血NK细胞（CD3⁻、CD16⁺）计数与健康受试者相比显著降低（137±11对229±13，p<0.001），并且观察到血液透析治疗持续时间与NK细胞（CD3⁻、CD16⁺）计数之间存在显著负相关（r=-0.391，p<0.05）。
1）慢性尿毒症和血液透析治疗对外周血NK细胞（CD3⁻、CD16⁺）计数有负面影响。2）接受血液透析治疗的慢性肾衰竭患者外周血NK细胞（CD3⁻、CD16⁺）计数可能是易感性感染和恶性肿瘤的预后标志物。
