Eleftheriadis Theodoros, Kartsios Charalambos, Yiannaki Efi, Antoniadi Georgia, Kazila Polizo, Pliakos Konstantinos, Liakopoulos Vassilios, Markala Dimitra
Nephrology Department, General Hospital of Serres, Serres, Greece.
Nephrology (Carlton). 2009 Aug;14(5):471-5. doi: 10.1111/j.1440-1797.2008.01041.x. Epub 2009 Mar 26.
Clinical and experimental data indicate a deficient immune response in haemodialysis (HD) patients. Natural killer-like (NKL) T cells express on their surface both the T-cell antigen receptor (TCR) and a diverse set of NK-cell receptors (NKR) and share properties of both T cells and NK cells. zeta-Chain phosphorylation is an early event that follows TCR activation or some NKR activation. The zeta-chain of both T cell and NK cells is downregulated in many chronic inflammatory states, HD included. In the present study, NKL T-cell percentage and zeta-chain expression in HD patients were evaluated.
Thirty-three stable HD patients and 30 healthy volunteers were enrolled into the study. NKL T-cell percentage and NKL T-cell zeta-chain mean fluorescence intensity (MFI) were evaluated with flow cytometry. The inflammatory markers C-reactive protein, interleukin-6 and tumour necrosis factor-alpha were measured in the serum by means of enzyme-linked immunosorbent assay.
All the evaluated markers of inflammation were increased in HD patients. In these patients, NKL T-cell percentage (1.71 +/- 1.69% vs 3.94 +/- 3.86%) and zeta-chain MFI (3.66 +/- 2.79 vs 7.03 +/- 7.91) were decreased.
NKL T-cell percentage and zeta-chain expression is decreased in HD patients. Taking into consideration the continuously increasing age of the HD patients and that normally NKL T-cell numbers increase with age counteracting the impaired T-cell and NK-cell function accompanying advancing age, the above NKL T-cell disturbances could contribute to the impaired immune response in this population. Measures towards alleviating chronic inflammation could partially restore NKL T-cell impairment.
临床和实验数据表明血液透析（HD）患者存在免疫反应缺陷。自然杀伤样（NKL）T细胞在其表面同时表达T细胞抗原受体（TCR）和多种自然杀伤细胞受体（NKR），并兼具T细胞和NK细胞的特性。ζ链磷酸化是TCR激活或某些NKR激活后的早期事件。在包括HD在内的许多慢性炎症状态下，T细胞和NK细胞的ζ链均下调。在本研究中，评估了HD患者中NKL T细胞百分比和ζ链表达情况。
33例稳定的HD患者和30名健康志愿者纳入本研究。采用流式细胞术评估NKL T细胞百分比和NKL T细胞ζ链平均荧光强度（MFI）。通过酶联免疫吸附测定法检测血清中的炎症标志物C反应蛋白、白细胞介素-6和肿瘤坏死因子-α。
HD患者中所有评估的炎症标志物均升高。在这些患者中，NKL T细胞百分比（1.71±1.69%对3.94±3.86%）和ζ链MFI（3.66±2.79对7.03±7.91）均降低。
HD患者中NKL T细胞百分比和ζ链表达降低。考虑到HD患者年龄不断增加，且正常情况下NKL T细胞数量会随着年龄增长而增加，以抵消衰老过程中T细胞和NK细胞功能受损的情况，上述NKL T细胞紊乱可能导致该人群免疫反应受损。减轻慢性炎症的措施可能会部分恢复NKL T细胞损伤。
