Anderson S L, Carton J M, Lou J, Xing L, Rubin B Y
Department of Biological Sciences, Fordham University, Bronx, New York 10458, USA.
Virology. 1999 Mar 30;256(1):8-14. doi: 10.1006/viro.1999.9614.
A cDNA encoding the human guanylate binding protein-1 (hGBP-1) was expressed in HeLa cells using a constitutive expression vector. Stably transfected clones expressing hGBP-1 exhibited resistance to the cytopathic effect mediated by both vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV) and produced less viral progeny than control cells following infection with these viruses. To study the role hGBP-1 plays in the IFN-mediated antiviral effect, cells were stably transfected with a construct expressing antisense RNA for hGBP-1. VSV infection of IFN-alpha-treated antisense RNA-expressing cells produced an amount of virus comparable to that produced in the parental cell line, while EMCV infection of the IFN-alpha-treated transfected cells and VSV and EMCV infection of the IFN-gamma-treated transfected cells produced far more virus than was produced in the parental cell line. These results demonstrate that GBP-1 mediates an antiviral effect against VSV and EMCV and plays a role in the IFN-mediated antiviral response against these viruses.
使用组成型表达载体在HeLa细胞中表达了编码人鸟苷酸结合蛋白-1（hGBP-1）的cDNA。稳定转染表达hGBP-1的克隆对水泡性口炎病毒（VSV）和脑心肌炎病毒（EMCV）介导的细胞病变效应具有抗性，并且在感染这些病毒后产生的病毒后代比对照细胞少。为了研究hGBP-1在IFN介导的抗病毒作用中所起的作用，用表达hGBP-1反义RNA的构建体稳定转染细胞。IFN-α处理的表达反义RNA的细胞感染VSV后产生的病毒量与亲代细胞系中产生的病毒量相当，而IFN-α处理的转染细胞感染EMCV以及IFN-γ处理的转染细胞感染VSV和EMCV后产生的病毒量比亲代细胞系中产生的病毒量多得多。这些结果表明，GBP-1介导针对VSV和EMCV的抗病毒作用，并在IFN介导的针对这些病毒的抗病毒反应中发挥作用。
