Heinen E, Bassleer R, Calberg C M
Beitr Pathol. 1976 Nov;159(2):207-18. doi: 10.1016/s0005-8165(76)80006-6.
Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity are concerned. DNA alone has no apparent effect on the fibroblasts. As shown by fluorescence microscopy, the lower toxicity of DNA-E.B. seems to be related to its mode of penetration into the cells.
体外培养的鸡胚成纤维细胞用溴化乙锭（E.B.）或DNA-E.B.复合物（DNA-E.B.）处理。E.B.（5微克/毫升）会引起形态改变和细胞死亡，抑制DNA合成和有丝分裂活性。就细胞结构、DNA合成和有丝分裂活性而言，DNA-E.B.（E.B. 5微克/毫升）对成纤维细胞的毒性较小。单独的DNA对成纤维细胞没有明显影响。如荧光显微镜所示，DNA-E.B.较低的毒性似乎与其进入细胞的方式有关。
