Heinen E
Virchows Arch B Cell Pathol. 1978 Mar 2;27(1):79-87. doi: 10.1007/BF02888985.
Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell degeneration; cis-Pt was not toxic under our experimental contitions. EB-DNA complex penetrates into the macrophages, is liberated from DNA in vacuoles, then diffuses into the cell and is highly cytotoxic. Cis-Pt-DNA complex also penetrates into the cells, but cis-Pt cannot be released from DNA, cis-Pt-DNA complex accumulates inside cytoplasmic vacuoles but has no cytotoxic activity.
小鼠腹腔巨噬细胞在体外培养，并用溴化乙锭（EB）或顺二氯二氨铂（II）（顺铂）处理。EB会引发强烈的细胞学改变和细胞变性；在我们的实验条件下，顺铂无毒。EB-DNA复合物穿透巨噬细胞，在液泡中从DNA释放出来，然后扩散到细胞内并具有高度细胞毒性。顺铂-DNA复合物也能穿透细胞，但顺铂不能从DNA中释放出来，顺铂-DNA复合物在细胞质液泡内积累但没有细胞毒性活性。
