Avila J L, Bretaña A, Avila A
Am J Trop Med Hyg. 1979 May;28(3):456-60. doi: 10.4269/ajtmh.1979.28.456.
Two different strains of mice (AKR and NMRI-IVIC) were inoculated intraperitoneally with the virulent Y strain of Trypanosoma cruzi, and then treated with the lysosomotropic ethidium bromide-DNA complex, according to several different treatment schedules. When animals were treated 48 hours after intraperitoneal inoculation with three intraperitoneal doses of EB-DNA no parasitemia was detected, even after 11 weeks, confirming previous results. However, when infection was allowed to become fully established, that is 3-4 weeks after inoculation, and then challenged with several different treatment schedules (with varied doses and timing of administration) we failed to cure established Chagas' disease, suggesting that the claim of effectiveness for this EB-DNA complex is limited to early Chagas' disease.
将两种不同品系的小鼠（AKR和NMRI-IVIC）腹腔注射克氏锥虫的强毒株Y，然后根据几种不同的治疗方案，用溶酶体亲和性溴化乙锭 - DNA复合物进行治疗。当动物在腹腔接种后48小时接受三次腹腔注射EB - DNA治疗时，即使在11周后也未检测到寄生虫血症，这证实了先前的结果。然而，当感染完全确立后，即在接种后3 - 4周，然后用几种不同的治疗方案（不同剂量和给药时间）进行挑战时，我们未能治愈已确立的恰加斯病，这表明这种EB - DNA复合物有效的说法仅限于早期恰加斯病。
