Schuster S, Dandekar T, Fell D A
Department of Bioinformatics, Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany.
Trends Biotechnol. 1999 Feb;17(2):53-60. doi: 10.1016/s0167-7799(98)01290-6.
Rational metabolic engineering requires powerful theoretical methods such as pathway analysis, in which the topology of metabolic networks is considered. All metabolic capabilities in steady states are composed of elementary flux modes, which are minimal sets of enzymes that can each generate valid steady states. The modes of the fructose-2,6-bisphosphate cycle, the combined tricarboxylic-acid-glyoxylate-shunt system and tryptophan synthesis are used here for illustration. This approach can be used for many biotechnological applications such as increasing the yield of a product, channelling a product into desired pathways and in functional reconstruction from genomic data.
合理的代谢工程需要强大的理论方法，如通路分析，其中会考虑代谢网络的拓扑结构。稳态下的所有代谢能力均由基本通量模式组成，这些模式是各自能够产生有效稳态的最小酶集。本文以果糖-2,6-二磷酸循环、三羧酸-乙醛酸循环联合系统以及色氨酸合成的模式为例进行说明。这种方法可用于许多生物技术应用，如提高产品产量、将产品导入所需途径以及从基因组数据进行功能重建。
