Ellenberg J, Lippincott-Schwartz J, Presley J F
Cell Biology and Metabolism Branch, Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
Trends Cell Biol. 1999 Feb;9(2):52-6. doi: 10.1016/s0962-8924(98)01420-2.
Green fluorescent protein (GFP) has become an important tool in cell biology and is widely used as a reporter for imaging intracellular proteins and structures in live cells. Recently, spectral variants of GFP with red- and blue-shifted fluorescence emissions have been characterized, opening the possibility of double labelling with two different-coloured GFP fusion proteins. This article reviews recent advances in this technique, with special emphasis on time-lapse imaging applications in living cells.
绿色荧光蛋白（GFP）已成为细胞生物学中的一种重要工具，并被广泛用作报告分子，用于对活细胞内的蛋白质和结构进行成像。最近，已对具有红移和蓝移荧光发射的GFP光谱变体进行了表征，这为用两种不同颜色的GFP融合蛋白进行双重标记开辟了可能性。本文综述了该技术的最新进展，特别强调了在活细胞中的延时成像应用。
