White J, Stelzer E
Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Trends Cell Biol. 1999 Feb;9(2):61-5. doi: 10.1016/s0962-8924(98)01433-0.
Cell biologists have used photobleaching to investigate the lateral mobility of fluorophores on the cell surface since the 1970s. Fusions of green fluorescent protein (GFP) to specific proteins extend photobleaching techniques to the investigation of protein dynamics within the cell, leading to renewed interest in photobleaching experiments. This article revisits general photobleaching concepts, reviews what can be learned from them and discusses applications illustrating the potential of photobleaching GFP fusion proteins inside living cells.
自20世纪70年代以来，细胞生物学家一直使用光漂白技术来研究细胞表面荧光团的侧向流动性。绿色荧光蛋白（GFP）与特定蛋白质的融合将光漂白技术扩展到细胞内蛋白质动力学的研究，引发了人们对光漂白实验的新兴趣。本文回顾了一般的光漂白概念，总结了从中可以学到的知识，并讨论了相关应用，展示了在活细胞内对GFP融合蛋白进行光漂白的潜力。
