Kurokawa H, Yamashita Y, Takeda S, Miura K, Murata T, Kajiyama M
Second Department of Oral and Maxillofacial Surgery, Kyushu Dental College, Kitakyushu, Japan.
Fukuoka Igaku Zasshi. 1999 Jan;90(1):6-13.
Few Data are available regarding expression of proliferating cell nuclear antigen (PCNA) versus overexpression of p 53 protein and prognosis for oral squamous cell carcinoma (O-SCC). This study evaluated the relationship between expression of PCNA and p 53 protein and certain clinical and pathologic parameters to prognosis in O-SCC. Scattered PCNA immunoreactive cells were found in 50 of 51 (98.0%) tumors. The mean PCNA-labelling index (PCNA-LI) of O-SCC was 34.7 +/- 12.0% (n = 50). The PCNA-LI was significantly high in O-SCC when compared to normal squamous epithelium (3.9 +/- 2.1%, n = 10). Altogether, 28 of 51 (54.9%) tumors showed nuclear immunoreactive for the p 53 protein. p 53-positive nuclei were clearly stained as granular or reticular in O-SCC. Microscopically, it appeared that the staining patterns of PCNA- and p 53-positive cells were quite similar. The mean value of PCNA-LI for the p 53-positive cases was 37.9 +/- 11.9% (n = 28), which was significantly higher than the 30.8 +/- 11.1% of the 23 negative cases. The survival rates at 3-year and 5-year for patients with p 53-negative O-SCC were 78.3% and 73.4%, respectively, while those for patients with p 53-positive O-SCC were 51.9% and 43.2%, respectively. No significant correlation could be found between PCNA-LI and T classification, N classification and Stage classification. The expression of p 53 was detected in 48.5% (16/33) of cases without regional lymph node metastasis, and 66.7% (12/18) with regional lymph node metastasis. There was a significant difference between PCNA-LI and p 53-positive cases, and prognosis. In PCNA-LI, the Grade 4 by mode of invasion was significantly higher than Grade 1, 2 and 3. The PCNA-LI and p 53-positive cases were significantly correlated with histological grading of malignancy. The present study shows a relationship between PCNA and p 53 protein. It also suggests that both PCNA and p 53 positive rates are the malignant potential of the O-SCC.
关于增殖细胞核抗原（PCNA）表达与p53蛋白过表达及口腔鳞状细胞癌（O-SCC）预后的可用数据较少。本研究评估了PCNA和p53蛋白表达与O-SCC某些临床和病理参数及预后之间的关系。在51个肿瘤中的50个（98.0%）发现了散在的PCNA免疫反应性细胞。O-SCC的平均PCNA标记指数（PCNA-LI）为34.7±12.0%（n = 50）。与正常鳞状上皮（3.9±2.1%，n = 10）相比，O-SCC中的PCNA-LI显著较高。总共51个肿瘤中的28个（54.9%）显示p53蛋白的核免疫反应性。在O-SCC中，p53阳性核明显染为颗粒状或网状。显微镜下，PCNA和p53阳性细胞的染色模式似乎非常相似。p53阳性病例的PCNA-LI平均值为37.9±11.9%（n = 28），显著高于23例阴性病例的30.8±11.1%。p53阴性O-SCC患者的3年和5年生存率分别为78.3%和73.4%，而p53阳性O-SCC患者的生存率分别为51.9%和43.2%。在PCNA-LI与T分类、N分类和分期分类之间未发现显著相关性。p53表达在无区域淋巴结转移的病例中为48.5%（16/33），在有区域淋巴结转移的病例中为66.7%（12/18）。PCNA-LI与p53阳性病例及预后之间存在显著差异。在PCNA-LI中，侵袭方式为4级的显著高于1级、2级和3级。PCNA-LI和p53阳性病例与恶性组织学分级显著相关。本研究显示了PCNA与p53蛋白之间的关系。它还表明PCNA和p53阳性率均为O-SCC的恶性潜能。
