Kurokawa Hideo, Zhang Min, Matsumoto Shinobu, Yamashita Yoshihiro, Tanaka Toshiko, Tomoyose Taiki, Takano Hirofumi, Funaki Katsuyuki, Fukuyama Hiroshi, Takahashi Tetsu, Sakoda Sumio
Department of Oral and Maxillofacial Surgery, Miyazaki Medical College, University of Miyazaki, Miyazaki, Japan.
J Oral Pathol Med. 2005 Nov;34(10):602-7. doi: 10.1111/j.1600-0714.2005.00358.x.
Although many histopathologic characteristics of oral squamous cell carcinoma (O-SCC) have been identified as prognostic factors, accurate, and unequivocal factors have not been clearly identified. The purpose of this study was to evaluate a potential association between the histologic grade of malignancy at the deep invasive front and the expression of Ki-67 antigen and p53 protein in O-SCC.
The expression of Ki-67 antigen and p53 at the invasive tumor front area of O-SCC was examined by immunohistochemistry of archived tissue from 62 cases. The mean age of patients was 60.7 years (range: 37-89) and the male-female ratio was 1.6:1 (38 men, 24 women). There were 20, 17, 14, and 11 cases classified as stage I to stage IV, respectively. The correlation between the intensity of immunostaining for Ki-67 antigen and p53 and the histologic grade of malignancy at the deep invasive front (invasive front grade, IFG) was analyzed. The expression of Ki-67 antigen and p53 in normal oral epithelia (10 cases) was also investigated.
The mean Ki-67 labeling index (LI) in the O-SCC samples was 32.8 +/- 12.0% (n = 62). The mean total score of IFG (IFG score) was 9.1 +/- 2.7 points (n = 62). There was a significant linear correlation between the IFG score and the Ki-67 antigen (gamma = 0.651, R2 = 0.596, P < 0.0001). Of 50 tumors examined, 27 (54.0%) exhibited p53-positive nuclear immunostaining. The staining patterns for Ki-67 antigen and p53 were similar. Both Ki-67-LI and p53-positive status were significantly correlated with the IFG scores.
The findings of this study demonstrate that overexpression of Ki-67 antigen and p53 at the deep tumor invasive front of O-SCC is associated with histologic grade of malignancy.
尽管口腔鳞状细胞癌（O-SCC）的许多组织病理学特征已被确定为预后因素，但准确且明确的因素尚未被清晰识别。本研究的目的是评估O-SCC深部浸润前沿的恶性组织学分级与Ki-67抗原和p53蛋白表达之间的潜在关联。
通过对62例存档组织进行免疫组织化学检查，检测O-SCC浸润肿瘤前沿区域Ki-67抗原和p53的表达。患者的平均年龄为60.7岁（范围：37 - 89岁），男女比例为1.6:1（38名男性，24名女性）。分别有20、17、14和11例被分类为I期至IV期。分析Ki-67抗原和p53免疫染色强度与深部浸润前沿的恶性组织学分级（浸润前沿分级，IFG）之间的相关性。还研究了正常口腔上皮（10例）中Ki-67抗原和p53的表达。
O-SCC样本中Ki-67标记指数（LI）的平均值为32.8 +/- 12.0%（n = 62）。IFG的平均总分（IFG评分）为9.1 +/- 2.7分（n = 62）。IFG评分与Ki-67抗原之间存在显著的线性相关性（γ = 0.651，R2 = 0.596，P < 0.0001）。在检查的50个肿瘤中，27个（54.0%）表现出p53阳性核免疫染色。Ki-67抗原和p53的染色模式相似。Ki-67-LI和p53阳性状态均与IFG评分显著相关。
本研究结果表明，O-SCC肿瘤深部浸润前沿Ki-67抗原和p53的过表达与恶性组织学分级相关。
