Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
J Oral Pathol Med. 2011 Oct;40(9):693-8. doi: 10.1111/j.1600-0714.2011.01032.x. Epub 2011 Apr 18.
Abnormalities in cell-cycle-controlling genes are important in the malignant transformation and proliferation of tumors. Among these genes, the tumor suppressor gene p53 is the most notable, and its mutations provide an indicator of tumor progression and prognosis. Proliferating cell nuclear antigen (PCNA) is a highly conserved nuclear protein that is expressed during cell replication and DNA repair. This study examined the expression of p53 and PCNA at the invasive front of oral squamous cell carcinomas (OSCC) by immunohistochemical staining, and investigated the relationship of these proteins to clinicopathological findings and prognosis.
Fifty-nine biopsy cases of OSCC were examined by immunohistochemical staining. Clinicopathological data were gathered and patient survival was analyzed.
The p53 labeling index (p53-LI) and PCNA labeling index (PCNA-LI) were examined at the invasive front of the tumors. A high p53-LI (p53+) was observed in 17 of the 59 cases (28.8%) and a high PCNA-LI (PCNA+) was observed in 28 of the 59 cases (47.5%). Among the modes of cancer invasion, many of the p53+/PCNA+ cases could be confirmed as highly invasive cancer (P < 0.05). In addition, the p53+/PCNA+ cases showed a high risk of tumor recurrence compared with the other expression forms, and patients with p53+/PCNA+ had a worse prognosis than those with the other expression forms. High labeling indices of p53 and PCNA are associated with poor prognosis in patients with OSCC.
We suggest that it is important to investigate the expression of p53 and PCNA at the invasive front of OSCC.
细胞周期调控基因的异常在肿瘤的恶性转化和增殖中起着重要作用。在这些基因中，肿瘤抑制基因 p53 最为显著，其突变提供了肿瘤进展和预后的指标。增殖细胞核抗原（PCNA）是一种高度保守的核蛋白，在细胞复制和 DNA 修复过程中表达。本研究通过免疫组织化学染色检测口腔鳞状细胞癌（OSCC）侵袭前沿的 p53 和 PCNA 的表达，并探讨这些蛋白与临床病理发现和预后的关系。
对 59 例口腔鳞状细胞癌活检病例进行免疫组织化学染色。收集临床病理资料并分析患者生存情况。
在肿瘤的侵袭前沿检测了 p53 标记指数（p53-LI）和 PCNA 标记指数（PCNA-LI）。在 59 例病例中，有 17 例（28.8%）出现高 p53-LI（p53+），有 28 例（47.5%）出现高 PCNA-LI（PCNA+）。在癌症侵袭的模式中，许多 p53+/PCNA+病例可被确认为高度侵袭性癌症（P<0.05）。此外，p53+/PCNA+病例的肿瘤复发风险高于其他表达形式，p53+/PCNA+患者的预后比其他表达形式差。p53 和 PCNA 的高标记指数与 OSCC 患者的不良预后相关。
我们建议研究 OSCC 侵袭前沿的 p53 和 PCNA 的表达情况非常重要。
