Grgicević D, Pistotnik M, Flego I
Bilt Hematol Transfuz. 1976;4(3):53-60.
By means of continous flow centrifuge the authors have obtained suspensions of granulocytes from the blood of healthy donors. Results of granulocytophereses are presented here with the main points in the therapy of granulocytopenic patients with granulocytes rich suspension. The mean volume of the obtained suspension was 418ml. and it contained 9,78 X 10(9) leukocytes. 70 percent of them were granulocytes, the said volume contained 48 ml of red blood cells. All the donors had been premedicated with i.v. dexamethason (4mg./kg body weight). No one of the 51 donors developed anaemia or hypoproteinemia. After the application of the protamin sulphate, however, two donors had face flushing breathlessness and gastric ache. These symptoms disappeared after the administration of corticosteroids. Although they are connected with the administration of protamin sulphate we can not explain them.
作者通过连续流动离心机从健康供体的血液中获得了粒细胞悬液。本文介绍了粒细胞单采术的结果以及用富含粒细胞的悬液治疗粒细胞减少症患者的要点。所获悬液的平均体积为418毫升，含有9.78×10⁹个白细胞。其中70%为粒细胞，上述体积含有48毫升红细胞。所有供体均预先静脉注射地塞米松（4毫克/千克体重）。51名供体中无一例出现贫血或低蛋白血症。然而，应用硫酸鱼精蛋白后，两名供体出现面部潮红、呼吸急促和胃痛。给予皮质类固醇后这些症状消失。虽然这些症状与硫酸鱼精蛋白的应用有关，但我们无法解释其原因。
