Patel R K, Trivedi A H, Roy S K, Bhatavdekar J M, Shah P M, Patel D D
Department of Cancer Biology, The Gujarat Cancer and Research Institute, Asarwa, Ahmedabad, India.
J Exp Clin Cancer Res. 1998 Dec;17(4):419-24.
Pan masala is a dry complex mixture of areca nut, catechu, lime, cardamon, unspecified flavouring agents etc., with (PMT) or without tobacco (pm). We have previously reported genotoxic potential of tobacco, areca nut and pan masala per se. An antigenotoxic effect of alpha-tocopherol (AT) and ascorbic acid (AA) against the PM/PMT induced genotoxic on Chinese hamster ovary (CHO) cells have been studied using chromosone aberration (CA) assay. AT and AA, per se, had no effect on CA frequency at the concentrations used in the present study. The short-term treatment of AT with aqueous extracts of PM/PMT yielded lower frequencies of CA as compared to the cultures treated with aqueous extracts of PM/PMT alone. However, a statistically significant reduction in CA frequency was observed with continuous treatment only. AA had no statistically significant protective effect except for continuous treatment with 10 ug/ml AA against the aqueous extract of PMT. The results indicate the possible use of AT to reduce the risk of oral cancer among PM/PMT chewers.
嚼烟是槟榔、儿茶、石灰、小豆蔻、未指明的调味剂等的干燥混合复合物，有烟草（PMT）或无烟草（pm）。我们之前已经报道了烟草、槟榔和嚼烟本身的遗传毒性潜力。使用染色体畸变（CA）试验研究了α-生育酚（AT）和抗坏血酸（AA）对PM/PMT诱导的中国仓鼠卵巢（CHO）细胞遗传毒性的抗原毒性作用。在本研究使用的浓度下，AT和AA本身对CA频率没有影响。与仅用PM/PMT水提取物处理的培养物相比，用AT与PM/PMT水提取物进行短期处理产生的CA频率较低。然而，仅连续处理时观察到CA频率有统计学上的显著降低。除了用10μg/ml AA连续处理PMT水提取物外，AA没有统计学上的显著保护作用。结果表明AT可能用于降低PM/PMT咀嚼者患口腔癌的风险。
