Mattila P, Knuuttila M, Kovanen V, Svanberg M
Institute of Dentistry, University of Oulu, Aapistie 3, SF-90220 Oulu, Finland.
Calcif Tissue Int. 1999 Apr;64(4):340-4. doi: 10.1007/s002239900629.
The effects of 5, 10, and 20% dietary xylitol supplementations on the biomechanical properties, histological architecture, and the contents of collagen, pyridinoline, and deoxypyridinoline in long bones of rats were studied. Tibiae were used for the three-point bending test, and femurs were used for the torsion and loading test of the femoral neck. The 10 and 20% oral xylitol administrations caused a significant increase of tibial stress, femoral shear stress, and stress of the femoral neck as compared with the controls. Parallel, but not significant, effects were also seen in the 5% xylitol supplementation group. No significant differences in strain or Young's modulus of the tibiae were detected between the groups. An increased shear modulus of elasticity in femurs was detected in the 20% supplementation group as compared with the controls. The histomorphometrical data for the secondary spongiosa of the proximal tibia revealed that trabecular bone volume was significantly greater in all dietary xylitol supplementation groups as compared with the controls. The bone volume increased along with increasing xylitol content. No significant differences between the groups were detected concerning the amount of collagen per dry weight of organic matrix, the concentrations of pyridinoline or deoxypyridinoline in collagen, or the ratio of these crosslinks. This suggests no xylitol-dependent selective changes in these structures of bone collagen. In conclusion, dietary xylitol supplementation in rats improves the biomechanical properties of bone and increases the trabecular bone volume dose dependently.
研究了饮食中添加5%、10%和20%木糖醇对大鼠长骨生物力学性能、组织学结构以及胶原蛋白、吡啶啉和脱氧吡啶啉含量的影响。胫骨用于三点弯曲试验，股骨用于股骨颈的扭转和加载试验。与对照组相比，口服10%和20%木糖醇导致胫骨应力、股骨剪切应力和股骨颈应力显著增加。5%木糖醇补充组也观察到类似但不显著的效果。各组之间胫骨的应变或杨氏模量没有显著差异。与对照组相比，20%补充组的股骨剪切弹性模量增加。胫骨近端次级松质骨的组织形态计量学数据显示，与对照组相比，所有饮食木糖醇补充组的小梁骨体积显著更大。骨体积随着木糖醇含量的增加而增加。各组之间在每单位干重有机基质的胶原蛋白含量、胶原蛋白中吡啶啉或脱氧吡啶啉的浓度或这些交联物的比例方面未检测到显著差异。这表明在骨胶原蛋白的这些结构中没有木糖醇依赖性的选择性变化。总之，大鼠饮食中补充木糖醇可改善骨的生物力学性能，并剂量依赖性地增加小梁骨体积。
