Division of Animal and Nutritional Sciences, West Virginia University, P.O. Box 6108, Morgantown, WV, 26505, USA.
J Bone Miner Metab. 2014 Jan;32(1):38-47. doi: 10.1007/s00774-013-0469-2. Epub 2013 May 19.
Resveratrol has gained popularity as an "anti-aging" compound due to its antioxidant and anti-inflammatory properties. Few studies have investigated the role of resveratrol supplementation in the prevention of age-related bone loss and skeletal disuse despite increased inactivity and age-related bone loss in the elderly. The objective of the study was to investigate the effect of resveratrol supplementation on disuse and age-related bone loss. Old (age 33 months) Fischer 344 × Brown Norway male rats were provided either trans-resveratrol (12.5 mg/kg bw/day) or deionized distilled water by oral gavage for 21 days. Rats were hindlimb-suspended (HLS) or kept ambulatory (AMB) for 14 days. Both femora and tibiae were collected. Bone mass was measured by dual-energy X-ray absorptiometry and bone microstructure was determined by micro-computed tomography. HLS of old male rats accelerated loss of bone mineral content, decreased trabecular bone volume per unit of total volume, and increased trabecular separation. Resveratrol supplementation ameliorated bone demineralization and loss of bone microarchitecture in HLS old male rats. The peak force measured by the three-point bending test was reduced (P = 0.007) in HLS/control compared to AMB/control rats. Resveratrol supplementation ameliorated HLS-induced loss of femur strength. Plasma osteocalcin and alkaline phosphatase was higher (P < 0.04) and C-reactive protein was lower (P = 0.04) in old male rats given resveratrol. The bone protective effects of resveratrol appeared to be mediated through increased osteoblast bone formation, possibly due to reduced inflammation. Based on the results, resveratrol supplementation appeared to provide a feasible dietary therapy for preserving the skeletal system during disuse and age-related bone loss.
白藜芦醇因其抗氧化和抗炎特性而作为“抗衰老”化合物而广受欢迎。尽管老年人活动减少和与年龄相关的骨质流失增加，但很少有研究调查白藜芦醇补充剂在预防与年龄相关的骨质流失和骨骼废用中的作用。本研究的目的是研究白藜芦醇补充对废用和与年龄相关的骨质流失的影响。提供了反式白藜芦醇（12.5 mg/kg bw/day）或去离子蒸馏水通过口服灌胃 21 天。大鼠进行后肢悬吊（HLS）或保持活动（AMB）14 天。收集股骨和胫骨。通过双能 X 射线吸收法测量骨量，通过微计算机断层扫描确定骨微结构。老年雄性大鼠的 HLS 加速了骨矿物质含量的丧失，降低了单位总体积的小梁骨体积，增加了小梁分离。白藜芦醇补充剂改善了 HLS 老年雄性大鼠的骨质脱矿和骨微观结构的丧失。三点弯曲试验测量的峰值力降低（P = 0.007）在 HLS/对照与 AMB/对照大鼠相比。白藜芦醇补充剂改善了 HLS 诱导的股骨强度丧失。给予白藜芦醇的老年雄性大鼠的血浆骨钙素和碱性磷酸酶升高（P < 0.04），C 反应蛋白降低（P = 0.04）。白藜芦醇的骨保护作用似乎是通过增加成骨细胞的骨形成介导的，可能是由于炎症减少。基于这些结果，白藜芦醇补充剂似乎为在废用和与年龄相关的骨质流失期间维持骨骼系统提供了一种可行的饮食疗法。
