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克氏锥虫dUTP酶的结晶及初步X射线衍射分析

Crystallization and preliminary X-ray diffraction of Trypanosoma cruzi dUTPase.

作者信息

Bernier-Villamor V, Camacho A, González-Pacanowska D, Cedergren-Zeppezauer E, Antson A, Wilson K S

机构信息

Instituto de Parasitología y Biomedicina 'López Neyra', Ventanilla, 11, PO 18006 Granada, Spain.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):528-30. doi: 10.1107/s090744499801035x.

DOI:10.1107/s090744499801035x
PMID:10089367
Abstract

Crystals of Trypanosoma cruzi dUTPase have been grown. Two different morphologies are observed, depending on the molecular weight of the PEG used as precipitating agent in the mother liquor, both having a hexagonal unit cell with similar dimensions. Complete X-ray diffraction data have been collected to low resolution for one of the forms. The space group is P6322, with unit-cell dimensions a = 134.15, c = 147.05 A. Peaks in the self-rotation function and the solvent content are consistent with two molecules of dUTPase per asymmetric unit.

摘要

克氏锥虫dUTPase晶体已生长出来。根据母液中用作沉淀剂的聚乙二醇(PEG)的分子量,观察到两种不同的形态,二者均具有尺寸相似的六方晶胞。已对其中一种形态收集了低分辨率的完整X射线衍射数据。空间群为P6322,晶胞参数a = 134.15,c = 147.05 Å。自旋转函数中的峰和溶剂含量与每个不对称单元中有两个dUTPase分子一致。

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