Nowicki C, Montemartini M, Hunter G R, Blankenfeldt W, Kalisz H M, Hecht H J
Instituto de Química y Fisicoquímica Bíologicas (IQUIFIB), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina.
Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):105-7. doi: 10.1107/s0907444997008019.
Tyrosine aminotransferase from Trypanosoma cruzi has been crystallized from PEG 4000 at pH 6.8. The crystals belong to the monoclinic space group P21 and have lattice constants of a = 59.1, b = 103.0, c = 77.8 A, beta = 113.1 degrees for a data set measured at 138 K. The presence of a non-crystallographic twofold axis together with a Matthews parameter Vm of 2.5 A3 Da-1 indicates that the asymmetric unit contains one dimeric molecule. The crystals diffract to at least 2.7 A and are stable in the X-ray beam in a shock-frozen state. Native data sets have been collected at temperatures of 285 and 138 K using a Siemens X1000 detector on a rotating-anode generator.
